- Lv H. (2019) RNA-Seq and transcriptome analysis of nitrogen-deprivation responsive genes in Dunaliella salina TG strain. Theoretical and Experimental Plant Physiology 31(1), 139-155.
Nitrogen is a critical macronutrient for plant growth and development, and Dunaliella salina has various physiological responses to nitrogen deprivation, such as accumulating large amounts of carotenoids in its chloroplasts. However, little is known about the underlying global molecular response of D. salina to nitrogen deprivation. Herein, we used the Illumina platform to interrogate which genes of D. salina respond to nitrogen deprivation. The RNA-seq libraries of D. salina TG cultured under nitrogen-replete and -depleted conditions for 7 days generated 87,160,704 valid reads with an average length of 124.94 bp. De novo assembly produced 73,329 transcripts with an average length of 801 bp and 47,283 unigenes with an average length of 675 bp. 5803 unigenes were assigned to 50 gene ontology (GO) terms belonging to three main categories. A total of 6016 unigenes with 671 enzyme commission numbers were assigned to 251 predicted KEGG metabolic pathways, and 6296 unigenes were categorized into 25 KOG classifications. A total of 27,306 CDS were predicted from the 47,283 unigenes. Analysis of transcription levels revealed that 2380 genes were upregulated and 747 were downregulated during nitrogen deprivation. These nitrogen-deprivation responsive genes and differentially expressed transcriptional regulator genes were identified by GO classification. The transcriptome data may serve as a reference for further analysis of gene expression and functional genomics studies, and will facilitate the study of carotenoid biosynthesis and nitrogen metabolism in chlorophytes at the molecular level.