Hybrid vigor contributes in a large way to the yield and quality of cotton (Gossypium hirsutum) fiber. Although microRNAs play essential regulatory roles in flower induction and development, it is still unclear if microRNAs are involved in male sterility, as the regulatory molecular mechanisms of male sterility in cotton need to be better defined.
In a recent study led by researchers from Henan Academy of Agricultural Science, two independent small RNA libraries were constructed and sequenced from the young buds collected from the sporogenous cell formation to the meiosis stage of the male sterile line Yu98-8A and the near-isogenic line. MiRNA sequencing revealed 1588 and 1536 known microRNAs and 347 and 351 novel miRNAs from male sterile and male fertile libraries, respectively. MicroRNA expression profiles revealed that 49 conserved and 51 novel miRNAs were differentially expressed. Bioinformatic and degradome analysis indicated the regulatory complexity of microRNAs during flower induction and development. Further RT-qPCR and physiological analysis indicated that, among the different Kyoto Encyclopedia Gene and Genomes pathways, indole-3-acetic acid and gibberellic acid signaling transduction pathways may play pivotal regulatory functions in male sterility.
Expression pattern analysis of miRNA5658 of MS and MF young buds by RT-qPCR
Relative expression was calculated by normalizing to the level of the 5S rRNA coding gene in RT-qPCR. SP, PO and ME represent the sporogenous cell, pollen mother cell formation and meiosis stages, respectively. Bars represent means + standard deviations, which were derived from three biological replicates (n = 3), and different letters above the bar indicate a significant differences at p < 0.05.
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References
X. Yang, Y. Zhao, D. Xie, Y. Sun, X. Zhu, N. Esmaeili, et al. (2016) Identification and Functional Analysis of microRNAs Involved in the Anther Development in Cotton Genic Male Sterile Line Yu98-8A Int J Mol Sci. 7;17(10) pii: E1677. doi: 10.3390/ijms17101677 [article]