High seed vigor is significant for agriculture, as low seed vigor hinders the popularization of sweet corn (Zea mays L. saccharata Sturt). To better understand the involvement and regulatory mechanism of miRNAs and how they might play a role with seed vigor, researchers from the China Jiliang University had small RNA libraries from seed non-artificial aged and artificial aged for 2 days generated using LC Sciences’ small RNA sequencing service. In their work, a total of 27 differentially expressed miRNAs were discovered, of which 10 were further confirmed by real-time quantitative PCR. Furthermore, targets of miRNAs were identified through LC Sciences’ degradome sequencing service. The degradome-seq data identified a total 7 of 1142 targets that were potentially cleaved by 131 miRNAs. Gene ontology (GO) annotations of target transcripts indicated that 26 target genes cleaved by 9 differentially expressed miRNAs might play roles in the regulation of seed vigor, such as peroxidase superfamily protein targeted by PC-5p-213179_17 play roles in oxidation-reduction process and response to oxidative stress. These findings provide valuable information to understand the involvement of miRNAs with seed vigor.
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References
S. Gong, Y. Ding, S. Huang, C. Zhu (2015) Identification of miRNAs and their target genes associate with sweet corn seed vigor by combined small RNA and degradome sequencing J. Agrc. & Food Chem. doi: 10.1021/acs.jafc.5b00522 [article]