Under natural conditions, some embryos cannot implant successfully because of the dysfunction of receptive endometrium (RE). Thus, it is imperative to study the molecular mechanisms involved in the formation of RE from pre-receptive endometrium (PE). In a recent study, researchers from Northwest A&F University collected endometrium from gestational day 5 (D5, PE) and gestational day 15 (D15, RE) dairy goats to analyze the transcriptome using strand-specific total RNA-Seq.
Greater than 120 million high-quality paired-end reads were generated and 47,616 transcripts were identified in the endometrium of dairy goats using total RNA-sequencing. A total of 810 mRNAs were identified between the RE and PE meeting with P-values < 0.05. Bioinformatics analysis of the differentially expressed genes revealed that a number of biological processes and pathways were potentially involved in the establishment of the RE, notably energy metabolism and amino acid metabolism. Furthermore, it is speculated that CXCL14, IGFBP3, and LGALS15 potentially participated in the development of endometrium. What’s more, putative SNPs, InDels and AS events were identified and analyzed in the endometrium.
In a word, this resulting view of the transcriptome greatly enhances the comprehensive transcript catalog and uncovers global trends in gene expression during the formation of receptive endometrium in dairy goats.
Scatter plot of the GO pathway enrichment analysis of DEGs with P-value< 0.05
The x-axis indicates the number of unique sequences assigned to a specific pathway, the y-axis indicates the GO pathway. The pathway enrichment statistics were performed by Fisher’s exact test, and with a corrected p value < 0.05 were considered the significant pathways.
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Total RNA Sequencing Service -Total RNA-Seq (Whole Transcriptome) sequencing captures a broader range of gene expression changes and enables the detection of novel transcripts in both coding and non-coding RNA species. Ribo-Zero ribosomal RNA reduction chemistry is used in place a poly-A tail selection which efficiently removes ribosomal RNA (rRNA) This process minimizes ribosomal contamination and optimizes the percentage of reads covering RNA species of interest…[Learn more…]
References
L. Zhang, X.R. Liu, J.Z. Liu, L. Ma, Z.Q. Zhou, Y.X. Song, , B.Y. Cao (2017) The developmental transcriptome landscape of receptive endometrium during embryo implantation in dairy goats Gene doi: 10.1016/j.gene.2017.08.026 [abstract]