VariantPro™ – Custom Amplicon Panels
LC Sciences offers a customized solution to high-throughput genome wide variant analysis. The VariantPro™ system is an innovative multiplex PCR based targeted sequencing method that accomplishes target selection, library preparation and molecular tagging in a simple one-step workflow.
The VariantPro targeted sequencing system offers cost-effective, minimal hands-on, ultra-high resolution sequence analysis, that enables accurate detection of rare variants. It utilizes novel and patent pending Relay-PCR™ and Omega-Primer™ technologies.
Ordering
Information
Technical
Information
| Description | The assay kit contains all the reagents required for capture of your custom targets and library preparation for next-gen sequencing |
| Capture Method | The VariantPro system incorporates novel Relay-PCR and Omega Primer technologies to form a one-step multiplex PCR based workflow. |
| Number of Targets | Up to 20,000 |
| Amplicon Length | Depends on sequencing platform |
| DNA Sample Source | Frozen tissue, whole blood |
| Input DNA Required | 10 ng per tube for human genomic DNA |
| Number of Tubes | 1-2 |
| Assay Time | 4-6 hours |
| Hands-on Steps | 1 |
| Hands-on Time | 5 minutes |
| Sequencing Platform | Illumina |
| Multiplexing Capability | Up to 3024 samples in one lane |
| Coverage Uniformity | > 99% at 0.2 x mean |
| Dropout Rate | 0 |
VariantPro Technology
VariantPro targeted sequencing system is based on a novel multiplex PCR technology that combines major innovations to facilitate simple operation and high performance in the preparation of targeted sequencing libraries. The innovations are:
- OmegaPrimer – a novel primer design that fundamentally improves primer specificity and yet is capable of sustaining certain local template variations such as SNPs.
- Relay-PCR – an elegant PCR method that combines multiple target capture and monoplex PCR amplification into a single reaction and therefore simplifies targeted sequencing library preparation.
- Molecular Tags – used to identify individual PCR template molecules. Relay-PCR and Omega Primer in combination provides a perfect vehicle to carry the molecular tags into amplicons.
Genomic DNA
Relay-PCR