MicroRNAs (miRNAs) are critically important in both normal neuronal development and neurological diseases. Although cerebral ischemia has been shown to alter the miRNA profiles of rats, the role of miRNA in the cornu ammonis 1 region of the gerbil hippocampus under ischemic tolerance has not been studied.
In the present study, researchers from Okayama University subjected Mongolian gerbils to one or three times the nonlethal dose of 2-min transient common carotid artery occlusion (tCCAO). LC Sciences’ miRNA microarray technology detected 251 miRNAs and the expression of seven of these in terms of ischemic tolerance. They were compared at different time points: 1 day, 7 days, 1 month, and 6 months. mmu-miR-15a-5p, related to neurogenesis, showed increased expression after one dose of 2-min tCCAO and was much higher after three doses. An increase in sha-miR-24 and oan-let-7b-3p, related to transactivation response DNA-binding protein (TDP43), was observed after one dose of 2-min tCCAO, but the peak was accelerated to an earlier period of reperfusion after three doses. In contrast, mmu-miR-125b-5p and mmu-miR-132-5p, related to fused in sarcoma/translocated in liposarcoma (FUS/TLS), showed similar increases at both doses. mmu-miR-181c-5p and mmu-miR-378a-5p, related to heat shock protein 70 (HSP70), also showed accelerated expression after three doses.
This data set provides new insight about miRNA expression during neurogenesis, and related to TDP43, FUS/TLS, and HSP70, which may be useful when pursuing further studies on the possible use of miRNAs as biomarkers in cerebral ischemic tolerance and neuroregeneration.
Time-dependent profiles of microRNA expression in gerbil hippocampus. Hierarchical clustering of miRNAs detected in the hippocampal CA1 region showed four distinct clusters for neuroprotection and protein expression. Each column represents a single sample, and each row represents a single miRNA. Black squares represent median level of miRNA, and green or red squares represent lower or higher levels than the median level of miRNA expression, respectively (A). Clusters B, C, D, and E contained miRNAs related to neurogenesis, 43 kDa transactivation response DNA-binding protein (TDP43), fused in sarcoma/translocated in liposarcoma (FUS/TLS), and heat shock protein 70 (HSP70), respectively. tCCAO, transient common carotid artery occlusion; mmu-miR, Mus musculus (mouse) microRNA; oan-let-7b, Ornithorhynchus anatinus (platypus) let-7d microRNA; sha-miR-24, Sarcophilus harrisii (Tasmanian devil) microRNA 24.
Related Service
miRNA Microarray Service – LC Sciences provides a microRNA (miRNA) expression profiling service using microarrays based on our in-house developed µParaflo® technology platform. We have standard arrays for all mature miRNAs of all species available in the latest version of the miRBase database (Release 21, July 2014). Our service is comprehensive and includes sample labeling, array hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we’ll send you both the raw and fully analyzed data. [Learn more…]
Reference
Sun M, Yamashita T, Shang J, Liu N, Deguchi K, Feng J, Abe K. (2015/03/31/) Time-Dependent Profiles of MicroRNA Expression Induced by Ischemic Preconditioning in the Gerbil Hippocampus. Cell Transplantation 24(3), 367-376. [abstract]