The VariantBaits targeted sequencing system was developed to meet the needs of researchers for larger-scale targeted gene screening.
The VariantBaits solution-phase hybridization capture system enriches the genomic target regions of interest to customers and performs high-throughput sequencing on next-generation sequencing platforms such as Illumina, Ion Torrent and BGI. Large-scale synthesis of high-quality oligonucleotides by LC Sciences’ proprietary μParaflo® microfluidic chip to prepare ultra-long RNA probes, liquid-hybridization of genomic libraries with sequencing adapters, and complementary pairing with target region sequences. The probe specifically binds to the DNA of the target fragment upon hybridization, and binds to the biotin label on the probe through the streptavidin magnetic beads, thereby capturing and enriching the DNA of the target fragment. Can be used for small to large-scale gene capture, tolerating the variation of certain partial templates.
Genomic DNA
Solution Hybridization
Sequencing
Efficient and cost-effective targeted sequencing strategy
Based on biotin-labeled RNA probe in-solution hybridization selection
Simple and flexible custom probe design solution
High quality oligos synthesized by LC Sciences patented μParaflo® microfluidics chip
Easy library prep and fast data analysis
Specific, sensitive large gene region capturing
High density probe coverage to generate excellent capture results
Less sequence data, higher target coverage
