We synthesize thousands of oligonucleotide sequences at once in parallel and then cleave the oligos releasing them into solution in a single microtube. Synthesis occurs via standard DMT chemistry assuring efficient stepwise yield and high quality final product. The product is delivered as a pool in a single microtube – ready to use for your experiment.
Ordering
Information
Technical
Information
| Product Description | mix of DNA oligonucleotide sequences |
| Number of Oligos | thousands of sequences or more per tube |
| Oligo Form | single stranded, desalted and ready for reactions |
| Length | up to 150mers |
| 5′ or 3′ terminus Modifications | phosphate, fluorescent dyes, biotin, linkers, and others (inquire) |
| Internal Modifications | modified DNA or RNA bases |
| Yield | *tens of attomoles per sequence and a total of sub-fmols per OligoMix® tube |
| Delivery | 14 days |
Selected Applications
Overview of the Service
Microfluidic Array Platform—in situ Synthesis
OligoMix® achieves high synthesis purity because it is produced via an advanced microarray synthesis technology (µParaflo®) that integrates a photo-generated acid (PGA) chemistry, digital photolithography (DLP), and advanced microfluidics to enable high throughput parallel synthesis of custom DNA microarrays. The PGA chemistry enables the use of standard oligo building blocks, and eliminates the need for any specially modified nucleotides which may exhibit lower coupling efficiency. DLP technology enables programmable synthesis of custom sequences and the µParaflo® microfluidic device contains the synthesis reactions each within a picoliter-scale reaction chamber, producing more uniform synthesis than reactions performed on the open surface of a slide.
- Conventional Chemicals
- Established Synthesis Processes
- Efficient Stepwise Yield
- Quality Final Product
Synthesis Technology References
- Gao X, Yu PY, LeProust E, Sonigo L, Pellois JP, Zhang H. (1998) Oligonucleotide synthesis using solution photogenerated acids. Journal of the American Chemical Society 120, 12698-12699 [abstract].
- Srivannavit O, Gulari M, Gulari E, LeProust E, Pellois JP, Gao X, Zhou X. (2004) Design and fabrication of microwell array chips for a solution-based, photogenerated acid-catalyzed parallel oligonucleotide DNA synthesis. Sensors and Actuators A. 116, 150-160 [abstract].
- Zhou X, Cai S, Hong A, Yu P, Sheng N, Srivannavit O, Yong Q, Muranjan S, Rouillard JM, Xia Y, Zhang X, Xiang Q, Ganesh R, Zhu Q, Makejko A, Gulari E, Gao X. (2004) Microfluidic picoarray synthesis of oligodeoxynucleotides and simultaneously assembling of multiple DNA sequences. Nucleic Acids Research 32, 5409-5417 [abstract].
- Tian J, Gong H, Sheng N, Zhou X, Gulari E, Gao X, Church G. (2004) Accurate multiplex gene synthesis from programmable DNA chips. Nature 432, 1050-1054 [abstract].