Single Cell RNA Sequencing Service

LC Sciences provides a fast single-cell RNA sequencing solution
from your single-cell suspensions or pre-made sequencing libraries
through data analysis.

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Home » Services » RNA Sequencing » Single-Cell RNA Sequencing Service
Technical Information

  • The latest in next-gen sequencing technology

  • Fast turnaround times

  • Full bioinformatics analysis

  • Expert technical support to assist with data interpretation

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Rapid Acceptance

Single-cell analysis has become one of the most interesting topics in contemporary biology and a rapidly growing field within the life sciences.

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Cell Heterogeneity

Single-cell analysis has become an established method to reveal cell heterogeneity and subpopulation expression and for rare cell characterization.

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True Expression

Understanding biological systems at a single cell resolution may reveal novel insights which remain masked by the conventional bulk RNA-based techniques.

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Novel Cell Types

Single-cell transcriptome sequencing holds the potential to identify novel cell types and characterize the cellular composition of any organ or tissue in health and disease

  • Demultiplexing of raw base calls

  • Performing alignment, filtering, barcode counting, and UMI counting

  • Generating gene-barcode matrices and determining clusters

  • Biostatistical analysis – expression analysis, multi-parameter data analysis, length distribution, transcript copy number comparisons, etc

  • Aggregating outputs from multiple runs and normalizing runs to the same sequencing depth (if applicable)

Sample Data Report

Cell Sample
Requirements

High-quality, viable cells
Depending on cell type,
freezing procedures need to be customized

Library Preparation

Library
Requirements

Any Illumina compatible library
Ex: 10X Chromium or BD Rhapsody

Sequencing Depth

Recommended
Sequencing Depth

100k reads per cell
(may vary based on cell type
and number of cells captured)

Quality

High
Data Quality

Data quality is measured by the percentage of bases with a sequencing quality score above Q30

Recent Discoveries
by our Customers

About Us
single cell rna sequencing

LC Sciences has been providing RNA discovery, profiling and related bioinformatics services since 2005 and our experts have examined thousands of varied RNA datasets, giving us unique insight into RNA research. We don’t merely deliver data; we will handle all the advanced bioinformatics analysis and help you find answers to the questions you’re asking.

Thank you so very very much for all of this incredibly helpful information and for replying to my request so quickly! I cannot tell you how much I appreciate your superior customer service!

Researcher - The Ohio State University

This report is Fantastic! I am very impressed.  You are in a competitive business and this really gives you an edge.

Researcher - Cal Poly State University

I wanted to let you know that I received the files, and that I’m very pleased with the quality and speed of delivery of the results. The service from LC Sciences has been outstanding.

Researcher - Case Western Reserve University

Thanks, the data quality looks great!

Researcher - Resphera Biosciences

We have been very satisfied with our miRNA microarray and are very much looking forward to the RNA-Seq results!

Researcher - Stanford University School of Medicine

I’m very happy to be your company client. During all this time (it’s been almost 10 years) you provided great service and always excellent support.

Researcher - The University of Bradford

My paper which was done with your great help has been published!

Researcher - Kyungpook National University
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Technical Information