Stress Response Studies
Identification of specific genes and miRNAs based markers that play essential roles in plant responses to both biotic and abiotic stressors
![stress Stress](png/stress-1.png)
Plant miRNAs have been shown to be involved in various biotic and abiotic stress responses
Plant miRNAs have been shown to be involved in various biotic (bacterial and viral pathogenesis) and abiotic stress responses such as oxidative, mineral nutrient deficiency, drought, salinity, temperature, cold (chilling), and other abiotic stress. miRNA expression profiling reveals that miRNAs which are involved in the progression of plant growth and development are differentially expressed during abiotic stress responses. The high-throughout techniques can provide genome-wide identification of stress-associated miRNAs under various abiotic stresses in plants.
miRNA microarray can monitor the expression pattern of thousands of genes simultaneously to determine microRNA profiling in stress-induced plant tissues/cells. The differentially expressed miRNAs further could be validated by quantitative real-time PCR (qRT-PCR) or by northern blot analysis. RNA-Seq technologies have become very powerful tools in the identification and quantification of small RNAs involved in gene regulation in different types of responses in plants and animals and degradome sequencing has revealed many novel plant miRNA targets1.
Agricultural Genomics – Stress Response
LC Sciences offers a portfolio of services to facilitate stress response studies in agricultural genomics. Contact us to find out more.
Experienced Service Provider – LC Sciences has been providing RNA discovery, profiling and related bioinformatics services since 2005 and our experts have examined thousands of varied RNA datasets, giving us unique insight into transcriptomics. We have sequenced over 75 different agricultural pest/pathogens, over 85 different plant species and over 100 different species of animals. Much of this data has been published in peer-reviewed journals.
Reference
1Kumar R. (2014) Role of MicroRNAs in Biotic and Abiotic Stress Responses in Crop Plants. Appl Biochem Biotechnol 174(1), 93-115 [abstract]